Table 2. Summary of the immunocytochemical detection of selected antigens in embryogenic callus.
Antibody | Positive (+) or negative (-) reaction | Signal localisation | ||
---|---|---|---|---|
Cell wall compartments | Internal cell compartments | Callus surface | ||
Arabinogalactan proteins | ||||
MAC207 | + | + | + | + |
JIM4 | - | - | - | - |
JIM8 | - | - | - | - |
JIM13 | + | + | - | - |
JIM16 | + | + | + | - |
LM2 | + | + | + | + |
Extensins | ||||
LM1 | - | - | - | - |
JIM11 | + | -* | - | + |
JIM12 | + | -* | - | - |
Pectins | ||||
LM19 | + | -* | - | + |
LM20 | + | +* | - | + |
LM13 | - | - | - | - |
LM16 | + | - | + | - |
LM6 | + | + | + | - |
LM9 | - | - | - | - |
Hemicelluloses | ||||
LM21 | + | - | + | - |
LM25 | + | + | - | - |
* signals were detected in the intercellular spaces for JIM11, JIM12, LM19 and LM20.