Figure 5.
Demonstration of pH decrease and acid generation.
Notes: (A) Acidic content increasing in the lysosomes as a function of exposure time in HCT-116 cells incubated with PEG–PAG9 (50 μM) for 24 h and with 1 μM of LysoSensor Green for 2 h after 5 s irradiation, 5.4 mW/cm2 (blue dots). Black dots represent cells incubated only with LysoSensor Green. (B) Increase in fluorescence intensity of LysoSensor Green in the presence of PEG–PAG9 at different irradiation times.
Abbreviations: PEG, polyethylene glycol; PAG, photoacid generator.