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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2016 May 24;73(6):271–285. doi: 10.1002/cm.21306

Figure 1. Diagram of PARF and PAAF method.

Figure 1

Before permeabilization of transfected cells, unbound labeled protein is in equilibrium with bound labeled protein. After permeabilization, the unbound labeled protein undergoes a very large dilution into the extracellular fluid, drastically decreasing the rate of label associating with the structure to such an extent that the dissociation rate becomes apparent (PARF). The permeabilization also allows access of soluble label from transfected cells to intracellular structures of untransfected cells, drastically increasing the rate of label associating with the structure to such an extent that the association rate becomes apparent (PAAF).