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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2016 May 24;73(6):271–285. doi: 10.1002/cm.21306

Figure 4. Cytoskeletal structures containing fluorescently labeled subunits continue to persist following 25µM digitonin permeabilization.

Figure 4

A) Nuclear (mchr-lamin A) and cytosolic (mchr-vimentin) intermediate filaments are both display a large insoluble fraction following permeabilization, indicating extremely slow exchange with soluble pools. B) The dissociation kinetics of GFP-labeled α-tubulin were slowed by stabilization with 10 µM taxol, where relative intensity levels never reach 50% of initial intensity due to a large immobile fraction (0.75±0.02) as a result of stabilization. Data points represent the sample mean ± SEM. For A), nlamin= 38 cells and nvimentin= 42 cells. For B), ntubulin=22 cells and n+taxol= 26 cells.