Table 1.
PARF kinetic analysis for GFP-tagged proteins used in this study
| Protein and condition | t1/2 | Immobile fraction |
R | n | ||
|---|---|---|---|---|---|---|
| Cells | Structures | Experiments | ||||
| GFP in cytosol | 11.7 ± 3.0 | 0.027 ± 0.042 | 0.998 | 16 | - | 5 |
| GFP in nucleus | 58.5 ± 6.8 | 0.004 ± 0.005 | 0.999 | 18 | - | 5 |
| GFP-H2B | - | 0.67 ± 0.03 | 0.998 | 32 | - | 6 |
| PM-GFP | - | 0.67 ± 0.08 | 0.998 | 17 | - | 6 |
| GFP-αtubulin | 29.5 ± 8.0 | 0.35 ± 0.06 | 0.995 | 22 | - | 8 |
| GFP-αtubulin + taxol | - | 0.75 ± 0.02 | 0.998 | 26 | - | 5 |
| 3xGFP-EMTB | - | 0.82 ± 0.03 | 0.943 | 23 | - | 6 |
| 3xGFP-EMTB + taxol | - | 0.85 ± 0.03 | 0.936 | 12 | - | 3 |
| GFP-actin | 26.1 ± 6.2 | 0.36 ± 0.02 | 0.991 | 36 | - | 7 |
| GFP-actin + jas | - | 0.62 ± 0.02 | 0.994 | 24 | - | 5 |
| GFP-LifeAct | 17.8 ± 3.9 | 0.06 ± 0.01 | 1.000 | 25 | - | 6 |
| GFP-LifeAct + jas | 11.8 ± 1.9 | 0.10 ± 0.13 | 0.989 | 29 | - | 6 |
| GFP-Ftractin | 16.2 ± 2.3 | 0.06 ± 0.01 | 1.000 | 42 | - | 9 |
| GFP-Ftractin + jas | 26.1 ± 4.7 | 0.09 ± 0.01 | 0.999 | 32 | - | 5 |
| GFP-Utrophin | 52.4 ± 10.5 | 0.31 ± 0.04 | 1.000 | 29 | - | 7 |
| GFP-Utrophin + jas | - | 0.63 ± 0.07 | 0.997 | 43 | - | 6 |
| GFP-VASP, whole cell | 20.7 ± 5.0 | 0.11 ± 0.02 | 0.998 | 23 | - | 8 |
| GFP-VASP, focal adhesions |
39.2 ± 3.3 | 0.21 ± 0.03 | 0.973 | 23 | 145 | 8 |
| GFP-VASP, filopodial tips | - | 0.71 ± 0.07 | 0.943 | 23 | 31 | 6 |