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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Immunol. 2016 Sep 4;310:141–149. doi: 10.1016/j.cellimm.2016.08.015

Fig. 1.

Fig. 1

IRF3 deficiency reduces T cell cytokine and Granzyme B expression during T cell responses with APCs. Primary splenic mononuclear cells (2.5 × 106) from C57Bl/6 and IRF3KO mice were stimulated with 1 µg/ml anti-CD3 with or without 1 µg/ml anti-CD28 with or without 25 µg/ml poly I:C for 24 or 48 h. Forty eight h supernatant levels of IFN-γ (A) and IL-17 (B) were assayed by ELISA, mRNA levels for GrB (C) and Foxp3 (D) were determined by qRT-PCR. Data are means ± SE, * indicates p< 0.05; NT indicates no treatment.