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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Immunol. 2016 Sep 4;310:141–149. doi: 10.1016/j.cellimm.2016.08.015

Fig. 3.

Fig. 3

INAM is induced during T cell responses and IRF3 contributes to T cell expression of cytokines and GrB. BM-derived DCs from C57Bl/6 (B6) or IRF3KO mice were incubated in cell culture with T cells (TC) from C57Bl/6 (B6) or IRF3KO mice (3 × 104 DCs with 3 × 105 TCs) and left unstimulated or stimulated with anti-CD3 with or without poly I:C (A–D) or stimulated with anti-CD3/anti-CD28 with or without poly I:C (E). After for 48 h supernatants were collected for IFN-γ (B,E) and IL-17 (C) ELISAs or total RNA isolated for INAM (A) and GrB (D) qRT-PCR. Data are means ± SE, * indicates p< 0.05 using two-tailed Student t test. Results shown are representative of two independent experiments (n = 3)