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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Dev Biol. 2016 Sep 12;419(2):262–272. doi: 10.1016/j.ydbio.2016.09.008

Figure 5. talin1 is required for CFC in zebrafish.

Figure 5

(A) talin1 is expressed within the POM and retinal/RPE cells lining the CF at 33hpf (arrow). (B,C) Lateral views of the eye of tln1hi3093Tg mutant (C) and wild-type sibling (B) at 3dpf. tln1 mutants possess colobomas. (D-F) Distal section through the eye of a 32hpf embryo demonstrating talin1 expression within the retina/RPE cells lining the CF (arrows) and the hyaloid vasculature (marked by GFP expression from fli1a:eGFP; arrowhead). (G-J) Sagittal sections through the eyes of 48hpf tln1 mutants and siblings stained with Lam-111 (red) and Sytox-green (DNA; green). BM degradation is disrupted in tln1 mutants at 48hpf. (K-N) Maximum projection images of the distal hyaloid in tln1 mutants and siblings demonstrating severe hypotrophy of the hyaloid in the tln1 mutant at 44.5hpf. Scale bar = 20μm (G-J, K,M) and 50μm (L,N).