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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Antiviral Res. 2016 Nov 5;136:51–59. doi: 10.1016/j.antiviral.2016.11.001

Fig. 3. O2-16 antiviral activity is dependent on A3G expression.

Fig. 3

(A) A western blot with an A3G antibody obtained through the NIH AIDS Reagent Program, Anti-Human APOBEC3G C-terminal Polyclonal from Dr. Jaisri Lingappa and a GAPDH antibody as a loading control showing the relative A3G expression in two CEM-derived cell lines, with low to no expression in CEM-SS vs. strong expression in A3.01. (B) CEM-SS and (D) A3.01 14-day HIVIIIB infections at low MOI were treated every other day with 0, 100, 200, and 400 nM of O2-16. RT activity was measured with radioactive nucleotides and measured by radioactive counts per minute (cpm) to determine relative HIV abundance in the media. 400 nM O2-16 neutralized HIV infection only in A3G expressing A3.01, n=3 with error bars showing SD. (C) CEM-SS and (E) A3.01 cytotoxicity relative to DMSO control at days 5 and 14 measured by tetrazolium dye, n=3 with error bars showing SD.