Table 1.
A3G Signature Hypermutations Identified through Next Generation Sequencing of HIV
| − Controla | O2-16 −Vif /−A3G |
O2-16 +Vif /+A3G |
+ Control −Vif /+A3G |
|
|---|---|---|---|---|
| Hypermutation Rateb | 1.0% | 3.1% | 81.8% | 97.1% |
| GG to AGc | 14.4% | 17.2% | 79.6% | 86.6% |
| GA to AAc | 36.1% | 35.4% | 16.8% | 11.7% |
| GT to ATc | 37.8% | 35.1% | 3.0% | 1.6% |
| GC to ACc | 11.7% | 12.3% | 0.7% | 0.1% |
| G mutations increased | 1 | 1.1 | 20.1 | 303 |
| STOP | --- | --- | 46.2% | 35.9% |
| MISSENSE | --- | --- | 40.3% | 49.7% |
| NO CHANGE | --- | --- | 13.5% | 14.4% |
a
HIV proviral DNA to control for mutations that arise during PCR and NGS
b
Among reads with at least 1 mutation, the % of reads containing 3 or more G mutations within an individual sequence read (150–200 bp read length).
c
% of total G mutations with nearest neighbor preference in an 885 bp region of the pol gene of 5.0 ×105 to 1.0 ×106 reads generated by NGS.
d
The fold-increase in G mutations compared to Negative (−) Control.