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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Reprod Toxicol. 2016 Oct 20;66:107–114. doi: 10.1016/j.reprotox.2016.10.004

Fig. 5.

Fig. 5

Effect of isoliquiritigenin on follicle mRNA level of regulators of steroidogenesis. Antral follicles were isolated from adult CD-1 mice and cultured with vehicle (DMSO) orisoliquiritigenin (ISL) for 48 and 96 h. Subsequently, follicles were pooled within experiment by treatment group and subjected to quantitative polymerase chain reaction for Cyp17a1 (panel A), Cyp19a1 (panel B), Hsd17b1 (panel C), and Star (panel D). Values were normalized to Actb. The graph represents the means ± SEM from 3 to 4 separate experiments. Asterisk (*) represents a significant difference between vehicle control and isoliquiritigenin (p ≤ 0.05).