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. 2016 Nov 18;39(11):797–806. doi: 10.14348/molcells.2016.0144

Fig. 3.

Fig. 3

The effect of insulin on lipogenesis and genes expression in ChREBP-siRNA porcine differentiated adipocytes. The ChREBP-siRNA and non-transfected adipocytes were shifted to serum-free medium for 12 h and transferred to basal medium containing 20 mmol/L glucose in the absence (open bars) or presence (grey bars) of 200 nmol/L insulin for 24 h. Lipogenesis and mRNA of specific genes were evaluated. ChREBP-siRNA means the cells transfected with pcDNA6.2-GW/EmGFP-ChREBP siRNA expression plasmid. Non-transfected indicates the unperturbed adipocytes. * (P < 0.05) and ** (P < 0.01) mean the significant between treatments in the same cell group. # (P < 0.05) and ## (P < 0.01) mean the significant between the same treatment in different cell groups. A: Quantification of lipogenesis in differentiated adipocytes by Oil Red O extraction. B-E: The relative mRNA level of LXRα (B), SREBP-1c (C), FAS (D) and ACC1 (E) measured by using real-time RT-PCR. Data are means ± SEM.