Fig. 8.

The role of LXRα in lipogenesis induced by insulin in porcine differentiated adipocytes. The ChREBP-siRNA adipocytes were shifted to a serum-free medium for 12 h, and transferred to the basal medium containing 20 mmol/L glucose with or without 1 μmol/L T0901317 in the absence or presence of 200 nmol/L insulin. After 24 h, the lipogenesis and the mRNA levels of these genes were measured. siRNA/FT means the adipocytes transfected with pcDNA6.2-GW/EmGFP-ChREBP siRNA expression plasmid were treated with a combination of the SREBP-1c inhibitor fatostatin (10 μmol/L) and T0901317. (A) Quantification of the cellular lipid content in differentiated adipocytes by Oil Red O extraction. (B, C) The relative mRNA level of FAS (B) and ACC1 (C) measured by using real-time RT-PCR. Data are means ± SEM.