Table 1.
The Primers of real-time PCR
| Gene* | Accession No. | Primer sequence (5′-3′) | Length, bp | Product size, bp |
|---|---|---|---|---|
| ChREBP | DQ372586.1 | F: GTATGTGGAGCGGAGGAAGAG | 21 | 93 |
| R: ATGACGGCCTCGGGTTT | 17 | |||
| SREBP-1c | AY307771.1 | F: TGAAGCCGGAGGTGGTAGA | 19 | 161 |
| R: CTTCATCTGGCTGTCCTCAAAA | 22 | |||
| LXRα | AB254406 | F:CTCGGACAGTCCCTTGGTAA | 20 | 85 |
| R: TCAGGAGAAACATCAGGCACA | 21 | |||
| FAS | EF589048.1 | F: AAGCAGGCGAACACGATG | 18 | 94 |
| R: GAAGGGAAGCAGGGTTGATG | 20 | |||
| ACC1 | EU168399 | R: AAGGGCTGCCTCTAATG | 17 | 95 |
| R: GATGTAAGCGCCGAACT | 17 | |||
| β-actin | AF054837 | F: GATCGTGCGGGACATCAA | 18 | 180 |
| R: AGGAAGGAGGGCTGGAAGAG | 20 |
*
ChREBP = Carbohydrate response element-binding protein; SREBP-1c = Sterol regulatory element-binding protein-1c; LXRα = liver X receptor alpha; FAS = Fatty acid synthase; ACC1=acetyl coenzyme A carboxylase 1