Fig. 2.

Inhibition of STAT3 elevates active β-catenin levels. (A) HEK293T cells were transfected with STAT3 siRNA (100 nM) or a scrambled control siRNA (100 nM, nonspecific siRNA used as a negative control). After treatment with a GSK inhibitor at a concentration of 1 μM for 4 h, cells were harvested, and the proteins were extracted in RIPA buffer for Western blotting. Anti-α-tubulin was used as a loading control. (B) HEK293T cells were transfected with β-catenin and then treated with 10 μM MG132 for 10 h before harvesting. Cells were incubated with 40 μM AG490 or a 10 μM STAT3 inhibitor overnight before harvesting. Cells were harvested, and the proteins were extracted in RIPA buffer for Western blotting. Anti-α-tubulin was used as the loading control.