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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Parkinsonism Relat Disord. 2016 Jul 20;31:79–86. doi: 10.1016/j.parkreldis.2016.07.009

Fig. 2. Cell and medium contents of catechols in rat pheochromocytoma (PC12) cells.

Fig. 2

The upper panel (A) shows a chromatograph of standards for DHPG = 3,4-dihydroxyphenylglycol, 1000 pg; NE = norepinephrine, 250 pg; DOPA = 3,4-dihydroxyphenylalanine, 1000 pg; EPI = epinephrine, 250 pg; DOPAL, 1000 pg; Cys-fluorodopa, 812 pg; DOPET = 3,4-dihydroxyphenylethanol, 1000 pg; DA, 250 pg; DOPAC, 1000 pg; Cys-DA, 787 pg; I.S. = internal standard (isoproterenol). The 10 catechols were baseline-resolved. The middle panel (B) shows a chromatograph corresponding to 400 μL of medium at 3 h of incubation. Note detection of endogenous DOPAL, DOPAC, and Cys-DA. The lower left panel (C) shows cell contents of Cys-DA after incubation with various concentrations of DOPA in the medium. Brown represents Cys-DOPA and blue Cys-DA; there are large increases in cell contents of Cys-DA but no change in Cys-DOPA. The lower right panel (D) shows cells contents of Cys-DOPA and Cys-DA after incubation with various concentrations of Cys-DOPA in the medium. There is no change in Cys-DA content despite large increases in Cys-DOPA content, indicating little if any conversion of Cys-DOPA to Cys-DA in the cells. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)