Figure 2.

TEM confirmation of nanoparticles internalization.

Notes: Top row: undifferentiated cells unlabeled and labeled with 15 μg Fe/mL CZF and PLL-coated γ-Fe₂O₃ (PLL) for 72 hours. Bottom row: differentiating cells unlabeled and labeled with 15 μg Fe/mL CZF and PLL-coated γ-Fe₂O₃ (PLL) for 72 hours. Insets show higher magnification views of nanoparticle clusters surrounded by membrane. Nanoparticle clusters are marked by arrows.

Abbreviations: TEM, transmission electron microscopy; CZF, silica-coated cobalt zinc ferrite nanoparticles; PLL-coated γ-Fe₂O₃, poly-l-lysine-coated iron oxide super-paramagnetic nanoparticles; N, nucleus; NF, neurofilaments.