Figure 6.

Immunocytochemical characterization of differentiating iPSC-NPs.

Notes: First column (A, D, G) represents unlabeled cells, second column (B, E, H) represents cells labeled with CZF (15 μg Fe/mL in cultivation media for 72 hours), and third column (C, F, I) represents cells labeled with PLL-coated γ-Fe₂O₃ (15 μg Fe/mL in cultivation media for 72 hours). Cells are stained for doublecortin (A–C), TH (D–F), synaptophysin (G–I) green and DAPI (blue).

Abbreviations: iPSC-NPs, induced pluripotent stem cell-derived neural precursors; CZF, silica-coated cobalt zinc ferrite nanoparticles; PLL-coated γ-Fe₂O₃, poly-l-lysine-coated iron oxide superparamagnetic nanoparticles; DCX, doublecortin; SYP, synaptophysin; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride.