Table 1.
ICP-MS measurement
| Nanoparticles | Cobalt (pg/cell) |
Zinc (pg/cell) |
Iron (pg/cell) |
|---|---|---|---|
| Unlabeled control | 0.00 | 0.13±0.18 | 0.13±0.18 |
| PLL-coated γ-Fe2O3 5 μg Fe/mL | – | – | 7.57±1.90 |
| PLL-coated γ-Fe2O3 10 μg Fe/mL | – | – | 18.04±5.26 |
| PLL-coated γ-Fe2O3 15 μg Fe/mL | – | – | 30.23±10.93 |
| CZF 5 μg Fe/mL | 0.93±0.45 | 0.88±0.83 | 3.21±1.49 |
| CZF 10 μg Fe/mL | 0.61±0.10 | 0.69±0.02 | 2.24±0.43 |
| CZF 15 μg Fe/mL | 0.71±0.14 | 0.79±0.02 | 2.82±0.87 |
Notes: Iron, cobalt and zinc content in unlabeled control iPSC-NP cells and in cells labeled with different concentrations of CZF and PLL-coated γ-Fe2O3 (5, 10 and 15 μg Fe/mL for 72 hours) measured by ICP-MS (n=3). Mean ± SD.
Abbreviations: ICP-MS, inductively coupled plasma mass spectrometry; PLL-coated γ-Fe2O3, poly-l-lysine-coated iron oxide superparamagnetic nanoparticles; CZF, silica-coated cobalt zinc ferrite nanoparticles.