Table 1.
Oligonucleotide primers used for the detection of tick-borne pathogens.
| Pathogen | Target gene | Oligonucleotide primer | Primer sequence (5′→3′) | Annealing temperature (°C) | Amplicon size (bp) | Reference |
|---|---|---|---|---|---|---|
| Anaplasma/Ehrlichia spp. | 16S rRNA | EHR16SD | GGTACCYACAGAAGAAGTCC | 52 | 345 | Cicuttin et al., 2014 |
| EHR16SR | TAGCACTCATCGTTTACAGC | |||||
| Anaplasma bovis | 16S rRNA | AB1F | CTCGTAGCTTGCTATGAGAAC | 54 | 551 | Kawahara et al., 2006 |
| AB1R | TCTCCCGGACTCCAGTCTG | |||||
| Anaplasma phagocytophilum | groEL | ANA-GroF | TCATTACTCAGAGTGCTTCTCAGTG | 55 | 372 | This study |
| ANA-GroR | CGATCAAACTGCATACCATCAGTC | |||||
| Ehrlichia spp. | groEL | gro607F | GAAGATGCWGTWGGWTGTACKGC | 55 | 730 | Tabara et al., 2007 |
| gro1294R | AGMGCTTCWCCTTCWACRTCYTC | |||||
| gro677F | ATTACTCAGAGTGCTTCTCARTG | 58 | 364 | |||
| gro1121R | TGCATACCRTCAGTYTTTTCAAC | |||||
| Babesia spp. | 18S rRNA | BJ1-F1 | GTCTTGTAATTGGAATGATGG | 53 | 1123-1173 | Casati et al., 2006 |
| BL-R1 | GAATAATTCACCGGATCACTCG | This study | ||||
| BJ1-F2 | GTCTTGTAATTGGAATGATGG | 58 | 750-820 | Casati et al., 2006 | ||
| BL-R2 | ATTAACCAGACAAATCACTC | This study | ||||
| Hepatozoon spp. | 18S rRNA | HepF300 | GCTAATACATGAGCAAAATCTCAA | 54 | 1131 | Sumrandee et al., 2015 |
| HepR900 | CGGAA TTAA CCAGACAAAT | |||||
| HepF | ATACATGAGCAAAATCTCAAC | 59 | 643 | Aydin et al., 2015 | ||
| HepR | CTTATTATTCCATGCTGCAG |