Table 1.

Name of necessary materials.

Materials	Company	Comments/description
1-gallon (3.8 L), wide-mouth, water jug	Coleman	MRI-compatible brain container with interior/exterior dimensions of ~5.75/8.00″ (W) × 5.75/8.25″ (D) × 9.75/11.75″ (H). (Available on Amazon or at most camping/outdoor retailers and department stores.)
Small bottle or tube of plastic epoxy	Any	To permanently seal the spout and any other openings on the lid of the MRI-compatible brain container. (Available on Amazon or at most hardware stores.)
A medium sized, water-tight, rectangular, plastic bucket	Any	Water/formalin overflow bucket. Interior dimensions should be at least as wide, approximately twice as deep, and ~9″ taller than the brain container (i.e., >8″ × ~16″ × ~22″). (Available on Amazon or at most department stores.)
Minimal expansion polyurethane insulating foam	Dow Chemicals Great Stuff™ Gaps and Cracks Insulating Foam Sealant	To fill the voids between the water/formalin overflow bucket and the MRI-compatible brain container. For full product details, please refer to the Canadian Construction Materials Center product report (CCMC 13074-L). (Available on Amazon or at most hardware stores and department stores.)
5-lb barbell weight plate	Any	To keep the MRI-compatible brain container submerged in the overflow bucket during water/formalin filling. (Available on Amazon or at most sporting goods stores and department stores.)
Duct tape	Any	To temporarily attach the 5-lb weight to the bottom of the MRI-compatible brain container during the water/formalin filling procedure. (Available on Amazon or at most hardware stores.)
Extra-large garbage or yard waste bags	Any	To place between the overflow bucket and the MRI-compatible brain container during water/formalin filling. Also to place around the MRI-compatible brain container in the MRI system in case of small drips/leaks from the lid. (Available on Amazon or at most grocery stores and department stores.)
Cotton batting	Any	To place inside the top and bottom of the MRI-compatible container (in order to pad the brain specimen). (Available on Amazon or at most sewing/fabric stores and department stores.)
Latex or nitrile surgical gloves	Any	To wear while handling brain specimens. (Available on Amazon or at department stores.)
Lab coats (or surgical gowns)	Any	To wear while handling brain specimens. (Available on Amazon or from most chemical supply companies.)
Multi-contrast MRI fiducial marker	Any (e.g., Beekley Medical MR-SPOTS Packets™)	To correctly identify right/left hemispheres in subsequent MRI data. (Available upon request in most radiology departments and MRI centers, but can also be purchased from a local medical supply company.)
Either stitches or string (and possibly a hemostat or tweezers to tie)	Any	To attach the fiducial marker to remaining dura matter or cerebral vein. (Available in any pathology department.)
Scissors	Any	To cut duct tape, stitches, yard waste bags, etc. (Available on Amazon, in most department and hardware stores, or in most pathology departments.)
Human MRI system and head coil (additional flex coil optional)	Any	To acquire whole-brain, ex vivo MRI data. (Need to request access from local radiology department or MRI facility.)
Small foam pad or folded sheet	Any	To place between posterior elements of the head coil and the MRI-compatible brain container (in order to hold the container securely in place and reduce vibrations during scanning). (Available in any MRI facility.)