Table 1.
Strain | Characteristic or genotype | Sources |
---|---|---|
C. jejuni NCTC12662 PT14 | propagating strain for bacteriophages CP_F1, CP220 | National Collection of Type Cultures |
C. jejuni NCTC12662 PT14 ΔflaA | ΔflaA::Kan | This study |
C. jejuni NCTC12662 PT14 ΔflaAB | ΔflaAB::Kan | This study |
C. jejuni NCTC12662 PT14 kpsM | kpsM::Kan | This study |
C. jejuni NCTC12662 PT14 flaB | flaB::Kan | This study |
C. jejuni NCTC12662 PT14 flaB, 0046::flaB | flaB::Kan, 00230::flaB cat | This study |
C. jejuni NCTC11168H flaA | ΔflaA::Kan | Jones et al., 2004 |
C. jejuni NCTC11168 flaAB | ΔflaAB::Kan | M. Jones Univ of Nottingham |
C. jejuni NCTC11168 flaB | flaB::Kan | M. Jones Univ of Nottingham |
C. jejuni NCTC11168 kpsM | kpsM::Kan | Karlyshev et al., 2000 |
E. coli TOP10 | Plasmid propagation strain | Invitrogen |
PLASMIDS | CHARACTERISTIC | |
pCfdxA | cj0046::cat | Gaskin et al., 2007 |
pC_flaB | cj0046::flaB cat | This study |
PRIMERS | NUCLEOTIDE SEQUENCE | |
flaA_fw | GATTGCACGATATAGCATTTAACAAG | |
flaA_rv | TCTAAACTAGGCTTGGATTTGTA | |
flaA_int_fw | AGGACTTGGAGCTTTAGCAGATGAGAT | |
flaA_int_rv | CGGCAGTATTAGCATCAAGCTGTC | |
flaA_i5′_rv | GTAAGATACCTAAAGCATCGTTAC | |
flaB_fw | GCCATGGCACAGGCTAATTC | |
flaB_rv | GGGTTTATGCACACGAAGCTTTGATAG | |
flaB_int_fw | AACGACCATAATTTTCCATCATATTTG | |
flaB_int_rv | GGTGAAGTGCAATTTACTCTTAAAAATTAC | |
kpsM_fw | AAGGTGTGCAAGCTAAGGCCGAGTT | |
kpsT_rv | GATCTCCAACAGCTCCTGCTTCAT | |
flaB_NgoMIV_fw | CGGCCGGCACAAATCCAAGCCTAGTTTAGAA* | |
flaB_BsmBI_rv | AACGTCTCACATGGACGAAGCTTTGATAGAAAATATCAT* | |
cj0046_fw | GCTCTTAGTGGCATTACCACTACC | |
cj0046_rv | GCCACACTAGTCGCATCAAGAGAA |
Restriction sites are underlined.