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. 2016 Nov 29;7:1908. doi: 10.3389/fmicb.2016.01908

Table 1.

Bacterial strains, plasmids, and primers used in this study.

Strain Characteristic or genotype Sources
C. jejuni NCTC12662 PT14 propagating strain for bacteriophages CP_F1, CP220 National Collection of Type Cultures
C. jejuni NCTC12662 PT14 ΔflaA ΔflaA::Kan This study
C. jejuni NCTC12662 PT14 ΔflaAB ΔflaAB::Kan This study
C. jejuni NCTC12662 PT14 kpsM kpsM::Kan This study
C. jejuni NCTC12662 PT14 flaB flaB::Kan This study
C. jejuni NCTC12662 PT14 flaB, 0046::flaB flaB::Kan, 00230::flaB cat This study
C. jejuni NCTC11168H flaA ΔflaA::Kan Jones et al., 2004
C. jejuni NCTC11168 flaAB ΔflaAB::Kan M. Jones Univ of Nottingham
C. jejuni NCTC11168 flaB flaB::Kan M. Jones Univ of Nottingham
C. jejuni NCTC11168 kpsM kpsM::Kan Karlyshev et al., 2000
E. coli TOP10 Plasmid propagation strain Invitrogen
PLASMIDS CHARACTERISTIC
pCfdxA cj0046::cat Gaskin et al., 2007
pC_flaB cj0046::flaB cat This study
PRIMERS NUCLEOTIDE SEQUENCE
flaA_fw GATTGCACGATATAGCATTTAACAAG
flaA_rv TCTAAACTAGGCTTGGATTTGTA
flaA_int_fw AGGACTTGGAGCTTTAGCAGATGAGAT
flaA_int_rv CGGCAGTATTAGCATCAAGCTGTC
flaA_i5′_rv GTAAGATACCTAAAGCATCGTTAC
flaB_fw GCCATGGCACAGGCTAATTC
flaB_rv GGGTTTATGCACACGAAGCTTTGATAG
flaB_int_fw AACGACCATAATTTTCCATCATATTTG
flaB_int_rv GGTGAAGTGCAATTTACTCTTAAAAATTAC
kpsM_fw AAGGTGTGCAAGCTAAGGCCGAGTT
kpsT_rv GATCTCCAACAGCTCCTGCTTCAT
flaB_NgoMIV_fw CGGCCGGCACAAATCCAAGCCTAGTTTAGAA*
flaB_BsmBI_rv AACGTCTCACATGGACGAAGCTTTGATAGAAAATATCAT*
cj0046_fw GCTCTTAGTGGCATTACCACTACC
cj0046_rv GCCACACTAGTCGCATCAAGAGAA
*

Restriction sites are underlined.