Figure 2.

Quantitative assessment of ureteric bud branching in mice with mutations in planar cell polarity genes. Quantitative analysis of the total and caudal/rostral ratios of ureteric bud (UB) branch tips (a,b), number of “segments” (i.e., sections between branch points plus the tips) in the tree (c,d), and number of branch points in the tree (e,f) (n = 3 for each genotype). mRNA levels of Celsr1 (g) and Vangl2 (h) in the caudal versus the rostral compartment of E13.5 wild-type metanephroi (n = 6) as assessed by quantitative reverse transcriptase-polymerase chain reaction. Data are presented as mean ± SEM. *P < 0.05 and **P < 0.01 between groups. Celsr1, Cadherin EGF LAG seven-pass G-type receptor 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Vangl2, Van Gogh-like 2.