Figure 4.

Assessment of glomeruli of E17.5 kidneys in mice with mutations in planar cell polarity genes. Representative images illustrating the 2 categories to which glomeruli were assigned: (a) Normal mature glomerulus and (b) immature glomerulus with <2 capillary loops. Quantification of glomerular morphology (c) showed a significant decrease in the proportion of normal glomeruli and an increase in the amount of immature glomeruli in Celsr1^Crsh/+:Vangl2^Lp/+ compared with wild-type mice. Assessment of tuft diameter (d) revealed no differences between any of the genotypes assessed. WT-1 and nephrin double immunostaining showed no difference in the glomerular expression pattern between wild-type, Celsr1^Crsh/+, Celsr1^Crsh/Crsh, and Celsr1^Crsh/+:Vangl2^Lp/+mice (e–h). Data are representative of n = 4–6. Bar = 50 μm in all panels. Data are presented as mean ± SEM. *P < 0.05 and **P < 0.01 between groups. Celsr1, Cadherin EGF LAG seven-pass G-type receptor 1; Vangl2, Van Gogh-like 2; WT-1, Wilms tumor-1.