Figure 1.

βIV-tubulin expression in PDA patient tissue and PDA cell lines. A) Immunohistochemistry for βIV-tubulin in A) human PDA tissue microarray specimens. B) Magnified field of human PDA tissue stained for βIV-tubulin. Panels show tissue stained with either isotype control antibody (i) or βIV-tubulin antibody (ii). The isotype control was negative and tumor elements had strong immunoreactivity for βIV-tubulin. C) Representative Western blot (n = 3) for βIV-tubulin in protein extracts from pancreatic cancer cell lines and human pancreatic ductal epithelial cells (HPDE). GAPDH was used as a protein loading control.