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. 2016 Nov 24;18(12):753–764. doi: 10.1016/j.neo.2016.10.011

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

The effect of βIVb-tubulin silencing on pancreatic cancer cell morphology and cell cycle. A and B) Confocal microscopy for α-tubulin and βIV-tubulin in MiaPaCa-2 (A) and HPAF-II (B) cells transfected with control siRNA (ns-siRNA) (top panels) or βIVb-tubulin siRNA (βIVb-Tub siRNA; bottom panels). Overlaid fluorescence images are shown in the far right panel of each row. C and D) Cell cycle distribution was analyzed by propidium iodide staining and flow cytometry. Bars represent % of MiaPaCa-2 (C) or HPAFII (D) cells in G0/G1-phase, S-phase, or G2/M-phase (mean ± S.E.M.); n = 3.