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. 2016 Nov 24;18(12):753–764. doi: 10.1016/j.neo.2016.10.011

Figure 9.

Figure 9

The effect of βIVb-tubulin silencing in pancreatic cancer cells on apoptosis. MiaPaCa-2 cells were transfected with control siRNA (ns-siRNA) or βIVb-tubulin siRNA (βIVb-Tub siRNA). 48 h later, cells were cultured in vincristine (0.1 nM, 0.3 nM, 0.5 nM) for a further 24 h. Apoptosis was then measured using a caspase 3/7 activity assay (A) or Annexin V and 7AAD staining and flow cytometry (B). Asterisks indicate significance relative to the 0 nM control for the same siRNA. Hashes indicate significance relative to the ns-siRNA control of the same drug dose. Asterisks indicate significance (* or #P < .05, **P < .01, *** or ### P ≤ .001, **** or ####P < .0001; n ≥ 3).