Table 1.
Significant metabolites identified by the pairwise comparison of baseline concentrations against 24 h at ~20 and ~9 °C
| Metabolite | Baseline (0 h) | 2 h | 8 h | 24 h | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean (µM) | Change [%) | P value | CV (%) | Change (%) | P value | CV (%) | Change (%) | P value | CV (%) | |
| ~20 °C | ||||||||||
| Arg | 17.40 | 1.0 | 0.82 | 5.8 | −28.3 | 0.03 | 17.9 | −40.9 | 3.1E−4 | 10.8 |
| Met | 12.65 | −7.1 | 0.26 | 5.1 | −15.3 | 0.16 | 18.0 | −43.7 | 1.3E−4 | 3.5 |
| Ser | 248.63 | −3.7 | 0.44 | 4.8 | −17.4 | 0.05 | 12.3 | −36.2 | 4.0E−5 | 6.6 |
| Val | 30.86 | −4.9 | 0.43 | 5.8 | −21.7 | 0.09 | 20.3 | −59.0 | 1.0E−5 | 3.5 |
| xLeu | 50.39 | −6.1 | 0.25 | 3.0 | −22.1 | 0.07 | 19.4 | −39.6 | 8.8E−5 | 3.6 |
| H1 | 622.83 | 3.7 | 0.19 | 2.2 | −3.0 | 0.67 | 15.0 | −30.3 | 9.5E−6 | 4.3 |
| ~9 °C | ||||||||||
| C6:1 | 0.16 | 1.9 | 0.77 | 9.2 | 2.2 | 0.76 | 10.3 | 16.2 | 8.1E−5 | 2.6 |
| Arg | 17.40 | −0.9 | 0.91 | 11.0 | 6.6 | 0.29 | 6.3 | −40.2 | 7.2E−5 | 7.0 |
| Val | 30.86 | −4.9 | 0.41 | 4.4 | −2.8 | 0.69 | 8.8 | −42.5 | 4.8E−5 | 8.1 |
| xLeu | 50.39 | −2.9 | 0.64 | 7.1 | 0.2 | 0.96 | 8.7 | −35.3 | 1.1E−4 | 4.9 |
The first column shows metabolites with significant changes in their concentration due to storage conditions when compared to the reference samples that are shown in the second column. The following columns show the percentage of concentration change, the respective P value, and coefficient variance (CV) derived with from the four measurements of the samples stored at the specified temperatures/conditions (2, 8, and 24 h at ~20 and ~9 °C). Significant P values (P < 7.9E−4) are indicated in bold