Table 3.

Metabolites significantly influenced by freeze and thaw cycles

Metabolite	Baseline (0 h)	Cycle 1 (26 h)			Cycle 2 (52 h)			Cycle 3 (78 h)
	Mean (µM)	Change (%)	P value	CV (%)	Change (%)	P value	CV (%)	Change (%)	P value	CV (%)
C3	1.00	1.3	0.55	2.3	7.7	0.13	7.1	13.8	7.7E−4	1.3
H1	622.84	4.7	0.24	5.6	12.2	0.07	8.3	20.2	3.1E−4	3.4

The first column shows metabolites that were significantly changed after the third freeze and thaw cycle when compared to the baseline values that are shown in the second column. The following columns show the percentage of concentration change, the respective P value, and coefficient variance (CV) derived with from the measurements of the sample after one, two, and three freeze and thaw cycles. Significant P values (P < 7.9E−4) are indicated in bold