Table 1.
The primer sequences for each of the genes
| Genes | Accession number | Primers Sequence a |
|---|---|---|
| IGF-1 | NM- 001082477 | F: AAG CCT ACA AAG TCA GCT CG R: GGT CTT GTT TCC TGC ACT TC |
| Bcl-2 | NM_016993.1 | F: F: CGGGAGAACAGGGTATGA R: CAGGCTGGAAGGAGAAGAT |
| GAPDH | NM_017008.4 | F: TGCCGCCTGGAGAAACCTGC R: TGAGAGCAATGCCAGCCCCA Target sequenceb |
| miR-29 | MIMAT0004718 | ACUGAUUUCUUUUGGUGUUCAG |
| miR-133 | MIMAT0017124 | AGCUGGUAAAAUGGAACCAAAU |
| miR-191a | MIMAT0000866 | CAACGGAAUCCCAAAAGCAGCUG |
a
Sequences were derived from NCBI (www.ncbi.nlm.nih.gov);
b
Sequences were derived from miRBase (www.mirbase.org)