Figure 3.

PCAIs inhibit pancreatic cancer colony formation and cell survival. Clonogenic cell survival assays were performed on MIAPaCa-2 cells treated with NSL-BA-055 and NSL-BA-056 (0-5 µM) for 48 h as described in the methods. A: Fluorescent images of the clones of survived cells were captured using an Olympus D70 microscope. B and C: Cell colonies with a minimum of 50 cells were counted and used to derive the cell survival fraction and cell survival plots as described in the methods. The results are the means (± SEM, N = 4) and are representative of four independent experiments. Statistical differences between control and treated groups were determined either by Dunnett’s post-test comparisons. Significance was defined as *P < 0.05; **P < 0.01 and ***P < 0.001.