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. 2016 Nov 1;6(11):2575–2586.

Figure 4.

Figure 4

USP22 restoration counteracted the suppressive effects of miR-101 on PTC cell proliferation and apoptosis resistance. K1 cells were transfected with miR-NC, miR-101 mimics, or miR-101 mimics + USP22-expressing plasmid. (A) Cell viability was measured by MTT assay. (B) The colony number was calculated. (C) The EdU-positive cells were counted to assess cell proliferation. Caspase-3 activity (D) and nucleosomal fragmentation (E) were determined. (F) The levels of USP22, cyclin D2, Rb, Bcl-2, Bax, cl-caspase-3, and caspase-3 were measured by western blot analysis. β-actin was used as endogenous control. All data are shown as means ± SD of three separate experiments. *P < 0.05, as compared with miR-NC group or miR-101 group; #P < 0.05, as compared with miR-101 group.