Figure 5.

USP22 overexpression reduced the inhibitory effects of miR-101 on PTC cell migration and invasion. K1 cells were transfected with miR-NC, miR-101 mimics, or miR-101 mimics + USP22-expressing plasmid. Transwell assays were performed to detect cell migration (A) and invasion (B), and the number of migrated and invaded cells was calculated. (C) Expression of USP22 and invasion-related proteins containing E-cadherin, vimentin, and snail was measured by western blot analysis. β-actin was used as endogenous control. All data are shown as means ± SD of three separate experiments. *P < 0.05, as compared with miR-NC group; #P < 0.05, as compared with miR-101 group.