Table 3.

Description of shared coding mutations identified in matched bilateral tumors and in peripheral blood samples (tissue panel)

Sample id	Gene	Mutation location	Coding	Protein	Function	MUT allele freq blood	MUT allele freq T1	MUT allele freq T2	COSMIC for the present mutation
BIL-006	FGFR3	T1, T2	c.1345C>T	p.Pro449Ser	Missense		0.52	0.5	Not registered
BIL-013*,$	TP53	T1, T2	c.818G>C	p.Arg273Pro	Missense		0.35	0.76**	COSM165077
BIL-017	PIK3CA	T1, T2	c.3140A>G	p.His1047Arg	Missense		0.3	0.22	COSM94986
BIL-028	ERBB2	T1, T2	c.2909G>A	p.Arg970Gln	Missense		0.63	0.55	Not registered
BIL-033	NOTCH1	T1, T2	c.6106G>A	p.Ala2036Thr	Missense		0.23	0.28	COSMIC: p.A2036V
BIL-035	MAP3K1	T1, T2	c.2012C>T	p.Ala671Val	Missense		0.41	0.48	COSM3674469
BIL-042*,$	TP53	BL, T1, T2	c.1021T>G	p.Phe341Val^	Missense	0.51	0.58	0.84**	Not registered, p.R342*; p.R342P
BIL-062	TP53	T1, T2	c.6061G>A	p.Val2021Ile	Missense		0.43	0.4	Not registered
BIL-064	MLL3	T1, T2	c.14732A>G	p.Asn4911Ser	Missense		0.51	0.64	Not registered
BIL-067$	TP53	T1, T2	c.856_857delGA	p.Glu286fs	Frameshift/Deletion		0.26	0.31	Not registered
BIL-020	FOXM1	BL	c.490C>T	p.Arg164Trp	Missense	0.47			COSM1476487
BIL-020	APOBEC3B	BL	c.568A>G	p.Arg190Gly	Missense	0.52			Not registered
BIL-048	EGFR	BL^^	c.3244A>T	p.Ile1082Leu	Missense	0.49			Not registered

^{^}shared TP53 mutation in blood (BL) and in both tumors (T1, T2).

^*metachronous cases, one with common blood/tumor TP53 mutation.

^$cases with germline mutations found upon testing for cancer predisposing genes.

^**increased VAF indicating loss of the wild-type allele in the metachronous tumor.

^{^^}non-informative amplicons and positions in the tumors of this case.