G3BP1 promotes HCC cell motility. A. Representative fields of YY8103 and Hep3B cells transfected with G3BP1 overexpression plasmid or empty vector in the transwell assay and average number of migration cells per field was evaluated. *P<0.05, **P<0.01. B. Representative fields of WRL68 and Sk-Hep-1 cells were evaluated by transwell assay after transfected with siG3BP1, where siNC was used as a control. Cell numbers were counted in five randomly selected microscopic fields. *P<0.05, **P<0.01. The overexpression and knockdown efficiency of G3BP1 was determined by western blotting. C. Wound healing experiment was used to analyze the motility ability of WRL68 and Sk-Hep-1 cells in which G3BP1 was silenced, photographs were taken at 0, 24, and 48 h.