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. 2016 Nov 1;6(11):2651–2660.

Figure 3.

Figure 3

CCAT2 promoted GC cells epithelial-to-mesenchymal-transition and interacted with EZH2, H3k27me3 and LSD1. A, B. Western-blotting analysis was used to detect the protein expression of Transcription factor ZEB2 and EMT markers (E-cadherin, N-cadherin and Vimentin) in si-CCAT2-transfected MKN45 cells or pcDNA-CCAT2-transfected BCG-823 cells. C, D. RNA levels in immunoprecipitates with EZH2, LSD1, and H3K27me3 were determined by qRT-PCR analysis in MKN45 and BCG-823 cells. Expression levels of CCAT2 were presented as fold enrichment relative to IgG immunoprecipitate. E, F. The levels of PTEN,P21, E-cadherin, LAST2,KLF2 and P15 mRNA were detected by qRT-PCR after knockdown of CCAT2 in MKN45 and BCG-823 cells. The mean values and S.D were calculated from triplicates of a representative experiment. **P < 0.05. G. The protein expression of LAST2 were detected by western-blotting analysis after knockdown of CCAT2 in MKN45 and BCG-823 cells.