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. 2016 Nov 1;6(11):2651–2660.

Figure 4.

Figure 4

CCAT2 interacted with EZH2, H3k27me3 and LSD1 and repressed the LATS2 expression. A, B. Chromatin immunoprecipitation-qPCR was used to analyze EZH2 and LSD1 occupancy, H3K27me3 binding to E-cadherin and LATS2 promoter regions in MKN45 and BCG-823 cells, C, D. Chromatin immunoprecipitation-qPCR was used to analyze EZH2 and LSD1 occupancy, H3K27me3 binding to the LATS2 promoter regions after knockdown of CCAT2 in MKN45 and BCG-823 cells, the IgG was acted as a negative control. The mean values and S.D were calculated from triplicates of a representative experiment. **P < 0.05. E, F. CCK8 cell proliferation assays were performed to evaluate the cell growth when transfected si-NC, si-CCAT2 or si-CCAT2+si-LATS2 in MKN45 cells or BCG-823 cells.