Figure 2.

TKT is a direct target of miR-497. (A) In silico algorithms predicted TKT contains one 7-mer putative miR-497-binding site on its 3’-UTRs. The mRNA (B) and protein (C) levels of TKTin miR-497 mimics-treated HeLa and SiHa cells were measured. β-actin was loaded as a control. (D) The direct physical interaction between miR-497 and TKT 3’-UTRs in HeLa and SiHa cells was demonstrated by luciferase reporter assay. (E) Immunohistochemical analysis of TKT expression in 74 cases of cervical squamous carcinoma. Oncomine data (F and G) demonstrated a significant increase in TKT expression in cervical cancer tissues compared to the normal control tissues. *P < 0.05, **P < 0.01. ***P < 0.001.