FIG 1.

RNF31 is cleaved during the process of apoptosis. (A and B) WB analysis of the indicated proteins in HeLa cells treated either with TNF-α (40 ng/ml) and CHX (10 μg/ml) (A) or with TRAIL (100 ng/ml) (B). Ex., exposure. (C) WB analysis of lysates of BxPC-1, Panc-1, A549, HCT116, HT29, and HeLa cells stimulated with TNF-α (20 ng/ml) and CHX (10 μg/ml) for 6 h. (D) WB analysis of HeLa cells exposed to Dox (3 μg/ml) or CPT (20 μM). (E) WB analysis of HeLa cells treated with a Smac mimetic (20 μM).