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RNF31 is cleaved by caspases during apoptosis but not during necroptosis. (A) Nonpretreated HeLa cells or HeLa cells pretreated with Z-VAD-FMK (20 μM) were treated with TNF-α (20 ng/ml) alone, CHX (10 μg/ml) alone, or both TNF-α and CHX and were then subjected to WB analysis. (B) WB analysis of WT, FADD-deficient, and caspase 8-deficient Jurkat cells stimulated with TNF-α (20 ng/ml) and CHX (10 μg/ml). Asterisks indicate pretreatment with the pancaspase inhibitor Z-VAD-FMK.
