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. 2016 Nov 28;36(24):3010–3018. doi: 10.1128/MCB.00474-16

FIG 5.

FIG 5

Activation of the NF-κB pathway is suppressed by RNF31 cleavage. (A) Schematic diagram of RNF31 domains. PUB, PNGase/UBA- or UBX-containing proteins; UBA, ubiquitin associated; IBR, in between Ring fingers; LDD, linear ubiquitin chain-determining domain. (B) Luciferase analysis of lysates from 293T cells transfected with NF-κB luciferase reporter genes and the indicated RNF31 mutants with or without HOIL-1 and Sharpin. FL, full length. (C) WB analysis of lysates from the cells analyzed in panel B. ubi, ubiquitination. (D) Luciferase analysis of lysates from 293T cells transfected with NF-κB luciferase reporter genes and the indicated RNF31 mutants with HOIL-1 and Sharpin. Con, control; n.s., not significant. Triple asterisks indicate significant differences (***, P < 0.001.).