FIG 1.

PDCD2L is a PRMT3-associated protein. (A) GFP-PRMT3 SILAC copurification results plotted by SILAC ratios on the x axis (intensity of peptides originating from the GFP-PRMT3 purification versus the control purification), which reflects signal specificity, and signal intensities on the y axis (total peptide intensity for each protein normalized to molecular mass), which reflects the relative abundance of each protein in the purification. Proteins presenting SILAC ratios of >2.0 and <2.0 are indicated. GFP-PRMT3, RPS2, and PDCD2L are shown in color. (B) Western analysis of total extracts (Input) (lanes 1 to 4) and anti-Flag immunoprecipitates (IP) (lanes 5 to 8) prepared from HEK 293 cells transfected with the empty vector (lanes 2 and 6), Flag-PABPN1 (lanes 3 and 7), and Flag-PDCD2L (lanes 4 and 8) constructs. The asterisk indicates the IgG heavy chain. Areas from the same blot were spliced to remove irrelevant lanes (between lanes 3 and 4 and between lanes 7 and 8). (C) Western blot (WB) analysis of immunoprecipitates prepared by using a PRMT3-specific antibody (lanes 3 and 4) and a control IgG antibody (lane 2), using extracts prepared from wild-type (lanes 1 to 3) and PDCD2L-null (1A3) (lane 4) cells. The asterisk indicates the IgG heavy chain.