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PDCD2L transits through the nucleolus. U-2 OS cells conditionally expressing GFP-PDCD2L were treated with LMB for 15 min (b, e, h, and k) and 90 min (c, f, i, and l) or left untreated (a, d, g, and j). Fixed cells were simultaneously analyzed by direct GFP fluorescence (g to i) and immunostaining for the nucleolar marker fibrillarin (d to f). DNA staining with DAPI shows the nucleus of each cell (a to c). Bar, 20 μm.
