FIG 6.

Analysis of pre-rRNA processing in PDCD2L-depleted cells. (A) Western blot analysis showing depletion of PDCD2L and bystin from HeLa cells. (B) Northern blot analysis of mature and precursor rRNAs using total RNA prepared from PDCD2L- and bystin-depleted cells as well as control cells. Pre-rRNAs were detected by using a probe complementary to sequences in the 5′ ITS (red probe in Fig. 3D) region. Pre-rRNA species are indicated on the left. Areas from the same blot were spliced to remove irrelevant lanes (between lanes 2 and 3). (C and D) 18S-E (C) and 21S (D) pre-rRNA levels were normalized to the 28S rRNA level and are expressed relative to levels in cells treated with control siRNA. Data and error bars represent the means and standard deviations, respectively, from 8 independent (siBystin) and 10 independent (siPDCD2L) experiments. **, P value of <0.01, as determined by Student's t test.