FIG 2.

Alloactivation enhances LFA-1-mediated adhesion to recombinant ICAM-1. (A) Syngeneically (syn) or allogeneically (allo) stimulated T cells and CD8⁺ naive T cells from the spleens of B10.A mice were incubated on plates coated with anti-human Fc antibody and then reincubated with recombinant mouse ICAM-1/Fc or buffer. After washing, bound cells were counted, and the adhesion was calculated by the ratio of bound cells to incubated cells. Error bars indicate standard error of the mean (SEM) (n = 10). (B) ICAM-1 adhesion of syngeneically (syn) or allogeneically (allo) stimulated CD8⁺ T cells after MLR was analyzed as described for panel A. Error bars indicate SEM (n = 10). (C) Expression of LFA-1 on syngeneically (syn) or allogeneically (allo) stimulated CD8⁺ T cells after MLR by flow cytometry. (D) Syngeneically (syn) or allogeneically (allo) stimulated T cells were incubated with anti-human IgG (negative control) or anti-mouse LFA-1 antibody (each at 20 μg/ml) for 30 min at 37°C, and then their adhesion to recombinant ICAM-1 was analyzed as described for panel A. Error bars indicate SEM (n = 20). ***, P < 0.001; **, 0.001 < P < 0.01.