FIG 7.

asp1^D333A cells require Mad2 for the prolonged delay into anaphase. (A) Serial dilution patch tests (10⁴ to 10¹ cells) of the indicated strains grown on YE5S plates without and with 8 μg/ml TBZ. The plates were incubated for 5 days at 25°C. (B) Diagrammatic illustration of the spindle length over time. A representative example was chosen for each of the indicated strains expressing SV40::GFP-Atb2⁺. (C) Quantification of the time required for the transition from prometaphase to anaphase B for the indicated strains (asp1^D333A mad2Δ strain, 19.3 ± 4.3 min; asp1^D333A strain, 36.2 ± 13.3 min; mad2Δ strain, 13.9 ± 2.0 min; n for all strains = 20. ***, P < 0.005 (two-sample t test).