TABLE 1.
S. pombe strains used in this study
| Strain | Genotype | Source (alternative strain name) |
|---|---|---|
| UFY2156 | h− SV40::GFP-Atb2+::LEU2 his3-D1 | Yeast Genetic Resource Center, Osaka, Japan |
| UFY1511 | h+ asp1D333A::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 | U. Fleig |
| UFY1579 | h+ asp1H397A::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 | U. Fleig |
| UFY2279 | h− asp1H397A::Kanr SV40::GFP-Atb2+::LEU2 | This study |
| UFY2280 | h− asp1D333A::Kanr SV40::GFP-Atb2+::LEU2 his3-D1 | This study |
| UFY605 | h− his3-D1 ade6-M210 leu1-32 ura4-D18 | K. Gould (425) |
| UFY857 | h− Kanr::nmt81::GFP-Atb2+ leu1-32 | T. Toda |
| UFY1763 | h+ asp1H397A::Kanr Kanr::nmt81::GFP-Atb2+ leu1-32 ura4-D18 | U. Fleig |
| UFY1529 | h+ asp1D333A::Kanr Kanr::nmt81::GFP-Atb2+ leu1-32 ura4-D18 | U. Fleig |
| UFY2249 | h− Sad1-mCherry::Kanr LacI-GFP::his7+ LacO-repeat::lys1+ ura4-D18 leu1-32 | This study |
| UFY2242 | h− asp1D333A::Kanr Sad1-mCherry::Kanr LacI-GFP::his7+ LacO-repeat::lys1+ | This study |
| UFY2357 | h+ ark1+-GFP::Kanr sid4+-mCherry::Natr | Silke Hauf (SK402) |
| UFY2363 | h− ark1+-GFP::Kanr Sid4+-mCherry::Natr asp1D333A::Kanr | This study |
| UFY1543 | h+ asp1D333A::Kanr mph1Δ::ura4+ his3-D1 ade6-M216 leu1-32 ura4-D18 | This study |
| UFY2337 | h− asp1D333A::Kanr mph1Δ::ura4+ SV40::GFP-Atb2+::LEU2 ura4-D18 | This study |
| UFY2367 | h− asp1H397A::Kanr Sad1-mCherry::Kanr LacI-GFP::his7+, LacO-repeat::lys1+ ura4-D18 leu1-32 | This study |
| UFY2646 | h− asp1D333A::Kanr mph1Δ::ura4+ LacI-GFP::his7+ LacO-repeat::lys1+ Sad1-mCherry::Kanr his− lys1-131 ura4-D18 leu1-32 | This study |
| UFY2627 | h− mph1Δ::ura4+ LacI-GFP::his7+ LacO-repeat::lys1+ Sad1-mCherry::Kanr leu1-32 ura4-D18 his− lys1-131 | This study |
| UFY138 | h− mph1Δ::ura4+ leu1-32 ura4-D18 ade6-M216 | Shalley Sazer |
| UFY103 | h− ade6-M210 leu1-32 ura4-D6 Ch16[ade6-M216] | Mitsuhiro Yanagida (10.2.2) |
| UFY2336 | leu1-32::SV40::Atb2+-GFP[LEU2] mad2Δ::ura4+ ura4-D18 | This study |
| UFY2335 | leu1-32::SV40::Atb2+-GFP[LEU2] asp1D333A::Kanr mad2Δ::ura4+ ura4-D18 | This study |
| UFY1790 | h− asp1D333A::Kanr ade6-M210 leu1-32 | This study |
| UFY139 | h− mad2Δ::ura4+ leu1-32 ura4-D18 ade6-M210 | Shelley Sazer |
| UFY2215 | h− asp1D333A::Kanr mad2Δ::ura4+ ura4-D18 leu1-32 | This study |
| UFY229 | h− cut7-466 leu1-32 | Iain Hagan (138) |
| UFY2422 | h+ klp6Δ::Kanr leu1 ura4 his2/7 | Takashi Toda (AR018) |
| UFY302 | h− pkl1Δ::his3+ leu1-32 ura4-D18 ade6-210 his3-D1 | Robin Allshire and Alison Pidoux (ALP192) |