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Icaritin induces caspase-dependent apoptosis in human GBM cell line U87. Assays are performed following treatment with Icaritin at indicated concentrations for 48 hours. A: Icaritin induces apoptotic cell death as measured by flow cytometry. B: Icaritin increases caspase-3 activity as determined by ELISA assay. C: Icaritin activates PARP and caspase-3c as assessed by western blot. D: Caspase inhibitor z-VAD-fmk abolishes icaritin-induced apoptosis as measured by flow cytometry.
