Figure 3.

Icaritin induces apoptosis via both intrinsic and extrinsic pathway in human GBM cell line U87. Assays are performed following treatment with icaritin at indicated concentrations for 48 hours. A: Icaritin activates caspase-8 and caspase-9 as determined by ELISA kit. B: Icaritin leads to loss of MMP as measured by flow cytometry following florescent staining. C: Icaritin causes release of cytochrome C from mitochondria to cytosol as assessed by western blot. D: Icaritin alters the expression of markers Fas for extrinsic apoptosis pathway as assessed by western blot. *P<0.05, **P<0.01.