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. 2016 Nov 15;8(11):4716–4725.

Figure 4.

Figure 4

NAC supplementation improves abnormal predentin maturation and dentin formation in Bmi1-/- mice. Paraffin embedded sections through the first molars from 5-week-old vehicle-treated wild-type (WT) and Bmi1-/- mice and NAC-treated Bmi1-/- mice stained with (A) HE, immunohistochemically for (B) biglycan and (C) dentin sialoprotein (DSP) and photographed. Scale bars represent 50 μm. (A) Representative HE staining of micrographs of the root walls of the first molars. (D) Quantitative thickness of predentin in the root walls of the first molars. (B) Representative micrographs of the root wall of the first molars stained immunohistochemically for biglycan, and (C) the root wall of the first molars stained immunohistochemically for DSP. (E) Quantitative biglycan immunopositive areas in the first molars. (F) Quantitative DSP immunopositive areas in the first molars. Each value is the mean ± s.e.m. of determinations in 5 animals of each group. **P<0.01; ***P<0.001 relative to the wild-type mice. #P<0.05; ###P<0.001 compared with Bmi1-/- mice.